Characterisation of locally isolated dye degrading microbe

The amount of textile effluent increases with the wide application of dye in the textile industry. Textile effluent produced from the textile industries contained a variety of dyes. The dye compound is known to be toxic in nature and haziardous. This causes soil and water pollution to the environment. There are several ways of treating the textile effluent, either chemically or physically. However, the high cost of the treatment had led to the urge of alternative ways, which is biological treatment. The purpose of this project is to study the degradation of the Reactive Orange 16 and Congo Red by isolate UMKDG-1. In this study, UMKDG-1 that isolated from the previous study was identified by molecular identification. 16S rRNA gene of UMKDG-1 was successfully amplified, purified and sent for sequencing. Then, the result of the BLAST analysis tool showed that UMKDG-1 has the highest similarity, 98% with Bacillus cereus. The result from the decolourisation assay showed that UMKDG-1 able to degrade 89.17% of 0.01 % Reactive Orange 16 and 57.64% of 0.01% Congo Red. The FTIR analysis showed that there are changes in the chemical structure of the Reactive Orangel6 and Congo Red that degraded by isolate UMKDG-1.