Nowadays, farming activities of Macrobrachium rosenbergii is one of importance sector around the world. The growth of human population will increase the production from industries and human activities. However, according to increasing the human activities a lot of heavy metals are generated. Heavy metals are harmful to most organisms especially for aquatic species even in low concentration. The mercury level in this species will be accumulated when this species consumed by their predators. This study was conducted to observe the survival rate of Macrobrachium rosenbergii against the effect of heavy metal of Mercury (Hg). The post larvae of Macrobrachium rosenbergii were exposed for four days in different concentration of mercury which is 0.01 ppb, 0.03 ppb and 0.05 ppb. The effect of heavy metal of mercury on mortality, resistance and bioaccumulation were studied. Survival of post larvae that exposed to 0.05 ppb were significantly lower than of those exposed to lower doses which are 0.03 ppb and 0.01 ppb. From this study, the result showed that when the concentrations of heavy metal of mercury increased, the percentages of mortality rates of giant freshwater prawns also increased which was 14.44% on 0.01 ppb, 28.89% on 0.03 ppb and 51.11% on 0.05 ppb. Hence, this research was conducted to investigate the effect of mercury toxicity on mortalities and resistance in giant freshwater prawn, M. rosenbergii. For bioaccumulation analysis, the result cannot be determined due to technical error. The finding of this research will provide a platform for future research and study on giant freshwater prawn against heavy metal.