Identification of Microbial Community in The Rearing Water Red Hybrid Tilapia (Oreochromis mossambicus x Oreochromis niloticus) Supplemented with Wood Vinegar

Red hybrid tilapia (Oreochromis mossambicus x Oreochromis niloticus) is an important aquaculture species for many countries and also commercially produced and consumed worldwide. Development of tilapia industry is significantly increased in Malaysia. However, the disease of tilapia affects the development of tilapia aquaculture and also cause the economic losses to the tilapia industry. In order to overcome the disease of tilapia, wood vinegar is applied as antimicrobial agent. Hence, study of the microbial community in the water of tilapia culture is necessary. Therefore, the detail of important microbial community in wood vinegar that play important role to overcome fish disease were revealed. In this study, water from the tilapia culture containing wood vinegar and the control without wood vinegar were cultured in the agar plate for pure bacteria isolation. The isolated bacteria were identified through Gram staining method, catalase test and oxidase test. In the tilapia culture containing wood vinegar and the control without wood vinegar, oxidase test showed oxidase negative and catalase test show catalase positive. From the Gram staining result, the result showed that there were more bacteria Gram negative compare to bacteria Gram positive but there are no significance. Further test for molecule analysis are required to carry out to further identify the bacteria species.