The purpose of the study is to investigate the effect of fermented soy pulp (FSP) and commercial fertilizer (NPK) on the growth performance of the freshwater microalgae culture.The fermented soy pulp was obtained at Prima Mekar Enterprise. The pond water that already filtered were put into a conical flask with different concentration mixed with the commercial fertilizer NPK (3g,6g and 9g) and the fermented soy pulp (FSP) (1g, 2g and 3g) (three replicate for each conical flask). The data were collected every morning at 7 days using the spectrophotometer reading (650 nm) and for the statistical analysis of the absorbance (AU) and the growth rate of the microalgae were done by using one way ANOVA and post hoc Tukey test at p<0.05 (SPSS version 23). At the end of the experiment, the FSP has the highest peak absorbance (AU) at (1.02±0.043 ) and the highest reading of the growth rate at (0.52±0.028 ). As the conclusion, FSP can be applied as the fertilizer in the freshwater microalgae culture replaced with the commercial fertilizer such as the NPK.