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Investigation of waste Banana Pseudo Stem (Musaacuminata) as potential raw material for Bioethanol production by fermentation process


Ramli, Nur Asma Faiza (2019) Investigation of waste Banana Pseudo Stem (Musaacuminata) as potential raw material for Bioethanol production by fermentation process. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)


Three different pre-treatment was used on the banana pseudo stem which is alkaline, acid and water pre-treatment was labelled as BS-Na0H, BS-H2SO4, and BS-H20. All three compound of treated banana was further undergo hydrolysis process in order to degrade the polysaccharides present in the pre-treated lignocellulosic biomass of banana waste into monosaccharide subunits. Monosaccharide that was produced through hydrolysis had enhanced the fermentation process by Saccharomyces cerevisiae. 10% of sulphuric acid was prepared and mixed with the lignocellulosic biomass of banana waste that was produced from three different pre-treatment process before. The glucose assay was carried out using Benedict's test to confirm the presence of reducing and non-reducing sugars before undergo fermentation process. The colour changes from benedict's reaction was collected at Oh, 24h, 48h, and 72h and this reaction gives a rough estimate of the reducing sugar present within the sample. As a result, shows a high reducing sugar for banana pseudo stern treat with alkaline and followed by banana pseudo stem treat with acid and water. FT-IR test shows an alcohol functional group, which is —OH bond in a broad band range 3600-3000cm-1 and SEM test shows a morphology of the banana pseudo stem had changes on the surface of sample after treated with different treatments. The highest percentage yield of ethanol produced is 16%. This shows that the best method to produce bioethanol from banana waste is to employ alkaline pre-treatment followed by acid hydrolysis and carry out the fermentation process.

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Additional Metadata

Item Type: Undergraduate Final Project Report
Collection Type: Final Year Project
Date: 2019
Call Number: SEH 2019 019
Supervisor: Dr. Nadiah Ameram
Programme: Forest Resource Technology
Institution: Universiti Malaysia Kelantan
Faculty/Centre/Office: Faculty of Bioengineering and Technology
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