Potential Antioxidant Activity of Ripe Pandanus tectorius Fruits Extract in Different Solvents

Pandanus tectorius is listed as underutilized crops in Malaysia and Indonesia and it fruit part is wasted and still not utilized either as a food source or for research. However, in Micronesia, Pandanus tectorius fruits have been used as major source of food but not fully exploited in South East Asia. The aims of the present study were to determined total phenolic content, total flavonoid content and potential of antioxidant from the keys parts of Pandanus tectorius fruits extract. The samples were collected from Kuala Nerus, Terengganu, Malaysia. Extracts were obtained by successive extraction using ethanol, acetone, ethyl acetate and petroleum ether as a solvent but petroleum ether could not be proceed as layered and cloudy was form in its mixture for each assay. The TPC and TFC were analysed by Folin-Ciocalteu and Aluminium Chloride Colorimetric assay respectively, meanwhile for antioxidant was analysed by DPPH free radical scavenging assay. The ethanol extract showed the highest TPC and TFC in result than other solvents where the result obtained is; ethanol (ethanol extract (4.52 ± 0.07 μg GAE / g raw material) and ethanol extract (29.42 ± 1.15 μg QE / g raw material), respectively. Result founded that both TPC and TFC of P. tectorius fruits were found to
be rich in phenolics content and flavonoids content as well as potent as antioxidant agent and the most suitable solvent for extraction of ripe Pandanus tectorius fruit is ethanol.