Bamboo is a multifunction plant because it can be used in construction, animal food, decoration landscape and any other usage. However, it become abundant and turns to be agriculture waste. Dyes are used in various types of industries such as textile, food, leather, paper and etc. Methyl Orange is one of dye that had been used in industry. As the industrial have develop and become more advance, the environment issues such as pollution become worse and need to be treated. The source of water supply usually had been polluted by the dye. Therefore, bamboos can be used as adsorbent for removal of Methyl orange (MO) dye. In this study, there are few parameters have been studied such as the effect of adsorbent particle size, adsorbent dosage, initial dye concentration, contact time and pH. The results showed that the adsorption of MO dye onto bamboo were optimum at adsorbent particle size of 0.125 mm, adsorbent dosage of 6 g, initial dye concentration of 150 mg/L, contact time of 24 hours and pH 7 with percentage removal of > 91 %. The equilibrium data was analysed using Langmuir and Freundlich adsorption isotherm model. The result showed that the equilibrium data was best fit to Langmuir adsorption isotherm model because the correlation coefficient is nearest to 1 and with maximum adsorption capacity was 1.688 mg/g. This study indicates that bamboo can be used as adsorbent for the removal of MO dye in water effluent.