Optimisation of proteus mirabilis strain PI18 β-Glucan extraction

Biopolymer, Microbial Polysaccharide (EPS) has showed promising benefit in various industries throughout last decade of study. However, the novelty and the complexity of the microbial EPS lead to drawback in exploiting this area. Previously, PI18 strain has shown promising β-glucan production through Scleroglucan base extraction method. In order to optimise further the β-glucan productivity, understanding the biosynthesis of βglucan from PI18 strain by subject the bacteria in nutrient stress and modifying previous extraction strategy to be time and cost efficient. This study has found that PI18 can biosynthesis β-glucan effectively in Minimal medium (Production media) and pH 7.00 in controlled environment (Bioreactor). Current study has achieved two days peak β-glucan production on Day 2-3 (1.412-1.424 mg/mL) by cultivation fresh seed culture. As compare with previous study only show peak β-glucan on Day 4 (2.752 mg/mL). Through modification of Scleroglucan base extraction strategy, the homogenization and three times dilution step can be eliminated to one-time dilution with distilled water can achieved better yield in shorter time.