Effect of BAP and NAA Concentration on Seed Germination and Growth of Cymbidium finlaysonianum orchid

Cymbidium finlaysonianum is an epiphytic orchid native in Malaysia. However due to deforestation and human exploitation, the distribution of this species in Malaysian forest has been decrease. The germination of this orchid in nature is difficult because it depends on specific mycorrhizal fungal symbionts that supply the carbohydrates in order to germinate. It is because orchids lack of endosperms. So, due to these difficulties an in vitro
seed germination is required in order to get the propagules. Therefore in this study, different growth media composition was used to enhance the germination Cymbidium finlaysonianum orchid seeds. Sterile orchid seed was cultured on seed germination medium supplemented with 1 mg/l, 2mg/l and 3mg/l of BAP respectively. The maximum seed germination with callus initiation was recorded in Dendrobium Seed Germination medium(DSG) with 3 mg/L of BAP . The protocorm was continuously observed using the Murashige and Skoog Medium (MS) media medium supplemented with 0 mg/L, 1 mg/l,
2mg/l and 3mg/l of BAP respectively and 0 mg/L and 1 mg/L of NAA respectively. Number of root, length of root, number of leaf, length of leaf, and height of plant were collected after 6 weeks to determine the best media for seed germination. The effect of different concentration of BAP was evaluated on PLB shows that 2 mg/L of BAP has been
proved as the best concentration on shoot development while MS basal (0 mg/L of BAP) for root development. After 6 weeks of observation,the plantlet of orchids was subcultured using MS media supplemented with 2 different concentration of NAA (0 mg/L and 1 mg/L of NAA). It is for determined the best concentration of NAA for root elongation.