Antibiogram Of Vibrio sp. Isolated from Semi-Closed System Farm Asian Clam, Corbicula fluminea

Vibrio sp. is gram-negative bacterium with facultative properties. They not only play as causative agent of vibriosis in clam farming but also lead to several disease for harming human health. Due to feeding feeder behaviour of Asian clam, the level of the Vibrio contamination on Asian clam tissues was studied. In Kelantan, Asian clam is known as ‘etok’ are popular among citizen and use to serve as snack or meal. This research provides an experimental study on Vibrio sp. isolated from Asian clam or also known as Corbicula fluminea. The objective of the study was to monitor the safety level of Vibrio contaminated via most probably number (MPN) analysis and antibiogram characterise of live Asian clam, Corbicula fluminea in semi-closed farm. Throughout the study, MPN test suggested it is not safe to be consume raw Asian clam. Vibrio colonies were
successfully isolated in Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and were subjected to 18 antibiotic discs for antibiogram test. Among all the antibiotics, nalidixic acid, oxolinic acid, oxytetracycline and tetracycline were found most sensitive followed by florfenicol (90%) and flumequine (70%) in controlling Vibrio sp. cultivated from water and tissue sample of Asian clam. Furthermore, the isolated bacteria identified by using BBL crystal enteric/nonfermenter kit confirmed the presence of V. Cholerae from the samples. Hence, mentioned antibiotics was advised to be used as prophylactic and treatment for clam farming while suitable treatment by applying appropriate temperature on clam cooking must conducted for safety consuming or preventing food poisonous.