Macrobrachium rosenbergii or also known as Giant Freshwater Prawn is crustacean species that can be found in Malaysia. The decreasing number of this crustacean species in the water streams make it one of luxurious food in the market. Some researchers have done the research on alternative feed of M. rosenbergii larvae in order to lower the feeding cost and improve the growth rate. Nevertheless, the ingredients used in the feed still needed some cost to be invested. This project was proposed to analyze the nutrition value of M. rosenbergii larvae formulated feed by using 40 % of egg custard, 3 % of egg shells, 1 % of Curcuma longa (Turmeric) and 17.5% of Moringa oleifera. There were 13 different feed formulations by using Response Surface Methodology (RSM) in
Design Expert Software version 10 which M. oleifera and C.longa were the variables coded while egg custard and egg shells were the based feed of the formulation. Proximate
analysis has been conducted to observe the nutrition composition of the formulated feed and compared with the nutrient requirement of M. rosenbergii larvae which were protein, lipid, mineral and carbohydrates requirement. The result by Design Expert Software version 10 revealed that M. oleifera and C. longa gave the significant effect to protein
and mineral requirement but not to lipid and carbohydrate requirement. The software also suggested the optimize formulation that near to the nutrient requirement of M. rosenbergii was Formulation 9, suggested percentage of 17.10 % M. oleifera and 1 % C. longa with 0.738 desirability.