Southeast Asia is a critical area for biodiversity and bats comprising nearly a third of mammal’s species in it. Gua Setir which located in Jeli, Kelantan is the suitable area to study the variety of bats since it is a limestone area and one of the main roosting areas for bats. This study provide the food habit of Rhinolophus affinis also known as Intermediate Horseshoe bat that captured in Gua Setir by using harp trap and mist net. The study was conducted to identify the order of insect in the Intermediate Horseshoe bat’s diet based on the faecal samples and the analysed the stomach content of this species. There are 20 samples of insect parts found in the sample from six order of insect. The orders listed are Diptera, Coleoptera, Lepidoptera, Blattodea, Hemiptera and Hymenoptera. In the end of study, Diptera were there highest order found comprising the head and thorax parts of the insect. This study also list out the left-over body part of insect. The body parts commonly found in the samples are the wings part of insects, the legs segments of insect and the antennae of the insects. This study provides useful baseline information about the diet analysis of this insectivorous bats species.