Effect of Extender Supplemented with Date Palm Pollen Grain on Bovine Semen Qualities

This study was to evaluate (1) The effect of extender supplementation with different concentration of date palm pollen grain (DPPG) on sperm quality parameter in Brahman
bull and (2) To observe the quality of sperm with different temperature between chilled and frozen semen after seven days of storage. The semen were collected through electrical
stimulation and assigned to four treatment groups. The semen were diluted in Tris citric fructose (TCF) based extender (control group;CG) and Tris based extender supplemented
with different concentration of DPPG (G1=2% DPPG; G2=4% DPPG and G3=6% DPPG). In the first experiment, the semen samples (n=12) were preserve in refrigerator (4°C) while in second experiment, the semen samples (n=12) were cryopreserve in liquid nitrogen and the experiment have three replication. The samples were thawed in water bath (37°C) and analysed for motility, membrane integrity and viability. The addition of 6% DPPG resulted in improved maintenance of sperm in chilling and frozen process. No significant different was observe among treatments groups for all the parameter (p>0.05) in first experiment. However, in the second experiment, the addition of 6% DPPG resulted significantly higher (p<0.05) in viability (71.25±1.04) compare to control groups (56.47±4.69). Besides, there is no significant different (p<0.05) of the concentrations on sperm motility. In conclusion, the supplementation 6% in 20 mg of DPPG had better ability to protect the bovine sperm in viability only while the supplementation of 20 mg for all concentration not quite effective to protect the sperm motility.