Investigation of light intensity, nutrient and carbon sources towards microalgae oil production via soxhlet extraction techniques

This study was carried out to study the optimized condition for microalgae cultivation in terms of light intensity, nutrient supply and carbon source to optimize the microalgae growth in order to produce high biomass with high lipid content. This is because to produce biodiesel, high microalgae lipid are needed which can achieve with a large amount of biomass. Thus, microalgae culture that labelled as MX1, MX2, MX3,MX4 was put under high light intensities, whereas MY1, MY2, MY3, MY4 were put under medium light intensity and MZ1, MZ2, MZ3 MZ4 will become control culture that been put under no light condition. The effect of the present of NPK fertilizers and presentof glucose will be observed simulated with light intensities parameter. At the end ofcultivation, MX2 obtained the highest biomass, and the biomass was being extractedwhich produced 97.186 g of biomass. The oil extraction yield is 9.66%. GC-MS analysis showed that the presence of UFA and PUFA in the oil. Thus, future research is needed to improve the technique to increase the microalgae biomass and lipid in the future in order to produced biodiesel.