This study was conducted to determine the population size of nectar bat (Eonycteris spelaea) at Gunung Reng, Kelantan, Malaysia since there have no information recorded. Estimated the population size of the E. spelaea at Gunung Reng, Kelantan, Malaysia was the main purpose of this study been conducted. Estimation by using photographic method had been set up about twenty days. The pictures were shoot five times for every roosts of bat in Gunung Reng which is there have 13 roosts was chosen due to the probability of getting good sampling data. The p-value<0.000, F = 5.661 with 95% confident interval, the population size of E, spelaea are between 207 to 344 individuals and the null hypothesis that the means are equal is rejected. In improving this research for the future there were a few factors that had been discussed which is the skill to control camera setting in every weather and environment, the bright source of light and the times interval between shoot of pictures.