This study examines the effectiveness of different Colony treatments on the growth of brood, honeypots, and beebread in Lepidotrigona terminata Colonies. Conducted from October 21, 2023, to January 1, 2024, for Colony 1, and from March 5, 2024, to May 7, 2024, for Colonies 2 and 3, the experiment measured weekly growth to assess treatment impacts. This study was conducted at Kg Paloh, Tendong, Pasir Mas, Kelantan. ANOVA results showed significant effects on brood growth (F = 17.057, df = 2, p < .001), with Colonies 2 and 3 outperforming Colony 1. However, honeypot and beebread pot growth showed no significant differences (F = 2.519, df = 2, p = .097), suggesting these aspects may be influenced by factors like temperature, humidity, and light intensity, requiring longer observation. Post hoc tests confirmed Colony 2 as the most favorable for brood growth, likely due to optimal environmental and structural conditions.