Microbial isolation and characterization of animal feed additives formulated using coconut water and rice water

Animal feed additives are rooted in the need to address challenges such as inefficient feed utilization, suboptimal animal growth, and health issues. These issues underscore the importance of developing and implementing effective feed additives to enhance digestibility, improve animal performance, and ultimately ensure the production of high-quality animal products while positively influencing the environment. This study aimed to isolate the microbial populations and characterize the animal feed additives produced from coconut water and rice water. The sources can be easily obtained from the surroundings and used as the substrate for the fermentation process. Microbial isolation and characterization were performed using various techniques such as serial dilution, spread plating agar, streaking, and gram staining. The fermentation broths were tested for their biochemical properties. The result of biochemical properties showed that protein content 0.092mg/ml which is higher in animal feed additives produced from coconut water compared to animal feed additives produced from rice water. The fermentation process took around 14 days approximately until constant pH was achieved. A glucose concentration test was also conducted and showed that both solutions have approximately the same glucose content, but animal feed additives produced from coconut water have slightly higher glucose content. In addition, antimicrobial tests were carried out and showed that the solutions have activity towards E. coli.