Acute Dermal Toxicological Study Of Chitosan/Polyvinyl Alcohol/Sodium Alginate Composite Film Loaded With Gynura Procumbens Extract As Potential Biofilm Plaster For Wound Healing

An abstract of the research paper presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement of the course DVT 55204 – Research Project.
This research sought to study the effects of novel biofilm containing Gynura procumbens, sodium alginate, chitosan, and PVA as prospective for wound dressings. The three different biofilms were constructed using solvent casting method and Soxhlet extraction method. Gynura procumbens extracts were incorporated in every biofilm. Each biofilm characterization includes the investigation of Acute Dermal Toxicity, Hematological analysis and histopathology. All rats were observed for any alterations in their physical characteristics and behavioural patterns that might arise as a result of the plant's toxic effects during the 14-day study period. Overall, the findings show that all three biofilms with Gynura procumbens extract equipped with chitosan, PVA, and sodium alginate-based films have promising potential as plasters for wound treatment in the future. The minimal changes that the biofilm caused indicates a new plausible plaster to be refined. The study emphasizes on the important aspects of appropriate film synthesis and presence of dermal and systemic toxicity that could pave a path for future development of wound healing plasters. Setting a foundation for further investigation and improvement for biofilms enhanced with Gynura procumbens for therapeutic applications, this research advances a potential for a new novel pharmaceutical product.
Keywords: Acute Dermal Toxicity, Polymer Based biofilm, Gynura procumbens, Wound healing plaster.