Occurrence Of Campylobacter Spp. Among Cattle In Kota Bharu Kelantan

An abstract of the research paper presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement of the course DVT 55204 – Research Project. This study aimed to investigate the prevalence of Campylobacter species among cattle in Kota Bharu, Kelantan, due to its zoonotic potential, which poses a risk of transmission to humans and can cause diarrhea in both cattle and humans. A total of 31 cattle were sampled, with vaginal and rectal swabs collected from females and rectal swabs from males. Samples were transported in Cary-Blair medium and cultured on modified charcoal cefoperazone deoxycholate agar (mCCDA) agar at 42 °C for 48 hours. The colonies were isolated for DNA extraction followed by Polymerase Chain Reaction (PCR). Various risk factors, including rearing system, age, sex, and previous treatments, were assessed. Data were analyzed using Microsoft Excel, employing descriptive statistics to determine the prevalence of Campylobacter species and evaluate potential influencing factors. Chi-squared tests were used to compare prevalence rates among different Campylobacter species and their sources. The results identified two positive cases from semi-intensive farms, emphasizing the rearing system as a significant contributing factor to the presence of Campylobacter species. These findings highlight the need for targeted management practices to mitigate zoonotic risks.