Assessing Antioxidant Level In Azadirachta Indica Methanolic Extract

An abstract of the research paper was presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement for the course DVT 55204 – Research Project. Reactive oxygen species (ROS) in excess can initiate and propagate various pathophysiological conditions, including inflammation, diabetes, genotoxicity, and cancer. Managing the oxidative damage induced by ROS is essential for reducing these health issues. Azadirachta indica, known for its therapeutic benefits, has potential as a natural antioxidant. This study aims to evaluate the antioxidant potential of methanolic extracts of A. indica, exploring its effectiveness in reducing or preventing the accumulation of free radicals in biological systems. Based on the results of the 2,2- diphenyl-1-picrylhydrazyl (DPPH) scavenging activity assay, methanolic extract showed lower antioxidant activity as compared to ascorbic acid. This is demonstrated by the IC50 values, where ascorbic acid exhibits antioxidants at 4 μg/mL, while the methanolic extract requires a significantly higher concentration of 49 μg/mL to achieve comparable activity. This suggests that A. indica demonstrates significant potential as an effective antioxidant for animals, capable of reducing the accumulation of harmful free radicals in their systems.
Keywords: Azadirachta indica, antioxidant level, methanolic extract