Analytical Profiling Of Azadirachta Indica Leaf Extracts Using Gas Chromatography-Mass Spectrometry

An abstract of the research paper was presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement for the course DVT 55204 – Research Project. All parts of the tree, from seeds, flowers, twigs, barks, roots, and leaves have their medicinal potential to humans. The efficacy of Azadirachta indica must be explored in order to determine its potential benefits. This study investigated the analytical profiling of methanol and hexane extracts of A. indica. Based on the gas chromatography-mass spectrometry (GS-MS), the methanol extract showed phosphoric acid, trimethyl ester, the most abundant compound, constituting 54.41% of the extract, followed by gamma-sitosterol (4.47%), hexadecanoic acid (3.11%), phytol (2.89%), and octadecanoic acid (2.39%). In hexane extract, phosphoric acid, trimethyl ester, also dominates with a slightly higher percentage of 61.36%. Hexadecanoic acid is the second most prevalent compound in hexane extracts at 5.14%, whereas gamma-sitosterol is present in a much lower proportion (0.45%). Octadecanoic acid and phytol were not detected in hexane extracts. These results provide valuable insights for future research focused on isolating and evaluating these compounds for their preservative properties.
Keywords: Azadirachta indica, analytical profiling, methanol, hexane