Molecular Detection And Characterization Of Vibrio Cholerae From Diseased Asian Seabass Using Multi-Gene Analysis

Vibrio cholerae is a gram-negative bacillus with a flagellum that can cause diarrheal diseases, cholera. Vibrio cholerae happens to be native aquatic environment habitat that may not cause infectious diseases in fish and aquatic animals but towards human. Thus, it is important to detect and characterize the Vibrio cholerae using multigene analysis. Two isolates of Vibrio cholerae were revived from the glycerol stock on TSA, CHROMagarTM and TCBS agar. Polymerase Chain Reaction was done using 11 primers which were pyrH, glp, gyrB, mdh, metG, purM, dtdS, lysA, pntA, pyrC and tnaA. The sequence information was compared with Genbank using Basic Local Alignment Search Tool (BLAST) (https://blast.ncbi.nlm.nih.gov/Blast.cgi). As a result, pyrH, gyrB, metG, purM, dtdS, pntA, and tnaA were detected from both V. cholerae isolates. By analyzing these genes, the molecular characterization of V. cholerae isolates became more robust, revealing genetic diversity and potential evolutionary adaptations among strains.
Keywords: Vibrio cholerae; Polymerase Chain Reaction; multi-gene analysis, housekeeping genes