A Study Of Bioactive Compounds Of Hibiscussabdariffa L. Extract Using Soxhlet Extraction Method

Hibiscus sabdariffa L. (H. sabdariffa) is known for delicacy and for medicinal p p H sabdariffa is in high demand worldwide due to it beneficial health propertierscOwertn:g. to bioactive compounds. However, the source of their bioactive compounds is litnited for therapeutic uses. The objective of this study are (1) to extract bioactive compounds from H. sabdariffa (calyces and leaves) using soxhlet extraction method; (2) to study the present of bioactive compounds from H. sabdariffa using phytoehemical screening test and; (3) to determine the effects of the type of solvents and ratio of the solid to solvent to the concentration of total phenolic, total flavonoid and total anthocyanin compounds. The H. sabdariffa calyces and leaves samples were extracted by using soxhlet extraction method with different type of solvents and different ratio of the solid to solvent. The pprhey,seonecheemofiebaiiosaccrteeivneincogmtepsotuwndess ocfonthdeucetedxtratcotss.wdeyrethar plyrseesednbcey oufs'mearg bFTohlRyciraTtehse, saponins, glycosides and tannins. The total phenolic, total flavonoid and total anthocyanin compounds of the Hibiscus sabdariffa extracts were determined by using UV-Vie spectrophotometer. The highest extraction yield of H. sabdariffa calyces and leaves extract using ethanol as solvent were found to be 10.59 ± 0.13 % and 4.090 0.00%, respectively. The concentration of total phenolic compound in H. sabdariffa calyceo extract was higher than leaves extracts using ethanol as solvent. The highest concentration of total phenolic content using ratio 1:2 (sample: solvent ratio) was found to be 0.237 mg/mL. The concentration of total flavonoid content in H. sabdariffa calyces extract was higher than leaves extract using water as solvent. The highest concentration of total flavonoid content in H. sabdariffa calyces ethanol extract with ratio 1:1(sample: solvent ratio) was found to be 0.0057 mg/mL. The concentration of total anthocyanin content in sabdariffa leaves extract was higher than calyces emract using ethanol as solvent. The highest concentration of total anthocyanin content in H.sabdariffa leaves ethanol extract with ratio 1:1 (sample: solvent ratio) was found to be 76.65 ± 0.00 mg/mL. Haibiescus sabdariffa calyces have therapeutic potential for commercialization as it hv interesting source of potential bioactive molecules with antioxidant, hypocholestero emtc antihypertensive, antimicrobial, anti-inflammatory, antidiabetic and anticarcinogenic activities.
Keywords: Hibiscus Sabdariffa L. , soxhlet extraction, bioactive compounds