Digital Special Collection Portal

Investigating the presence of key amino acid residues for human ace-2 receptor binding domain (rbd) of sars-cov-2-like coronaviruses isolated from bats in east coast malaysia


Citation

Ashley Joyce Poon (2023) Investigating the presence of key amino acid residues for human ace-2 receptor binding domain (rbd) of sars-cov-2-like coronaviruses isolated from bats in east coast malaysia. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)

Abstract

An abstract of the research paper submitted to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial fulfilment of the course DVT55204 – Research Project.

Covid-19 is a recently emerging form of severe acute respiratory syndrome (SARS) caused by SARS-Coronavirus-2 (SARS-CoV-2) which was first reported in Wuhan, China in December 2019. Bats are natural reservoirs for coronaviruses, and bat coronaviruses have been highlighted as some strains of SARS-like coronavirus (SARSL-CoV-2) carried by bats are almost identical to human SARS-CoV-2 at the genome level, especially the spike protein. Some strains of these bat coronaviruses are highly similar to human SARS-CoV-2 and are able to use the same receptor as SARS-CoV-2 to mediate cell entry. Early last year, five SARS-CoV-2-like viruses were isolated from bats in East Coast Malaysia (four from Sekayu, Terengganu and one from Gunung Reng, Kelantan). Angiotensin-converting enzyme 2 (ACE-2) has been identified as the important receptor of SARS-CoV and SARS-CoV-2 to enter human cells. The interaction between the S1 protein of the virus, particularly amino acid fragment 318-510, with the human ACE-2 receptor is the critical determinant of coronavirus host range and cross-species transmission. This study aimed to investigate the presence of key amino acid residues for human ACE-2 on the RBD of the spike protein of these bat coronaviruses. Reverse transcription polymerase chain reaction (RT-PCR), and sequencing of the PCR products were used to investigate the presence of the ACE-2 RBD. From the results obtained in this experiment, it was determined that coronaviruses isolated from bats in East Coast Malaysia can utilise human ACE-2 receptors to infect human cells and thus may become a public health concern in the future.

Keywords: SARS-CoV-2-like coronaviruses, ACE-2 receptors, Receptor Binding Domain (RBD), bat coronaviruses, human transmission

Download File / URL

[thumbnail of D19A0003 ASHLEY JOYCE POON.pdf] Text
D19A0003 ASHLEY JOYCE POON.pdf

Download (1MB)

Additional Metadata

Item Type: Undergraduate Final Project Report
Collection Type: Final Year Project
Date: 2023
Number of Pages: 43
Call Number: DVT 033
Supervisor: Prof. Dr. Maizan binti Mohamed
Programme: Doctor Of Veterinary Medicine
Institution: Universiti Malaysia Kelantan
Faculty/Centre/Office: Faculty of Veterinary Medicine
URI: http://discol.umk.edu.my/id/eprint/14158
Statistic Details: View Download Statistic

Edit Record (Admin Only)

View Item View Item

The Office of Library and Knowledge Management, Universiti Malaysia Kelantan, 16300 Bachok, Kelantan.
Digital Special Collection (UMK Repository) supports OAI 2.0 with a base URL of http://discol.umk.edu.my/cgi/oai2