Detection Of Trypanosoma Spp. In Asian Swamp Eel, monopterus Albus In Selected States In Malaysia

An abstract of the research paper was presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement for the course DVT 55204 – Research Project. Trypanosomiasis is one of the most important economic diseases affecting both marine and freshwater fish. Trypanosoma is a flagellated blood protozoan transmitted via leech parasitism in fish. Clinical signs in fish may manifest as emaciated, dullness, respiratory distress, mild ascites and anaemia. Asian Swamp Eels (Monopterus albus) are native to the tropical and subtropical regions in Asia and have the potential to become an economic aquatic commodities in Malaysia. This study aimed to detect the presence of Trypanosoma spp. in the swamp eel population across different locations in Kelantan, Terengganu and Perak. A total of 39 eels were collected from diverse locations each representing a diverse habitat of rivers, paddy fields and swamps. Detailed microscopic examination of the blood samples collected revealed no presence of trypanosomes. DNA extraction was done on the blood samples using the PrimeWay Genomic DNA Extraction Kit to obtain Trypanosoma genetic material. Kin1 and Kin2 primers were used in this study to detect the presence of Trypanosoma spp. The PCR analysis targeting specific Trypanosoma genes showed negative results for all the tested samples. As a result, no PCR products were forwarded for sequencing. The absence of trypanosomes detected may suggest a low risk of the diseases within these regions. However, it is essential to consider variations in environmental factors, habitats, and the dynamic nature of our aquatic ecosystems have influenced the study outcomes. Despite the negative results, the study contributes valuable insights into overcoming potential economic repercussions for Malaysia's aquaculture industry.
Keywords: Trypanosoma, Asian Swamp Eel, Malaysia, PCR Analysis