Serological detection of leptospirosis among dogs in kelantan

Leptospirosis is a zoonotic bacterial disease affecting both humans and animals. The aim of this study is to determine the seroprevalence of leptospirosis among dogs in Kelantan and to identify the most prevalent Leptospira serovars in dogs in Kelantan. A total of 30 serum samples from dogs were collected from private clinics in Kelantan and Hospital Pengajaran Veterinar, Universiti Malaysia Kelantan. Thirteen male dogs and 17 female dogs’ blood were collected using cephalic venepuncture. Samples were tested using Enzyme-Linked Immunosorbent Assay (ELISA) and Microscopic Agglutination Test (MAT). Among the dogs, 10% classified as young, 73% were adult and 17% were senior dogs. Based on the critical value of 0.1625 for ELISA, all of the dogs were tested negative for Canine Leptospira IgG. The cut-off antibody titre for MAT was ≥ 1:100, and all the samples were negative for 06 serovars tested for the MAT panel. The absence of Leptospira specific antibodies among study cohort carries substantial implications for public and animal health. The negative findings regarding Leptospira play a crucial role in contributing essential information to the local disease epidemiology, indicating a potential low risk of canine leptospirosis. These findings able to provide basis for the formulation of preventive strategies against canine leptospirosis in Kelantan, Malaysia. In conclusion, there were 0% seroprevalence of leptospirosis among dogs that enrolled for the study in Kelantan.
Keywords: Leptospirosis, Dog, Serology, ELISA, MAT