Evaluation of antioxidant activity of active film prepared with Chitosan and Sacha Inchi (Plukenetla Volubilis) leave extract

Sacha inchi (Plukenetia volubilis L.) leaves were popular used to make tea product but there is no research on the sacha inchi incorporated with chitosan film. Hence, the purpose of this study was to develop novel active films with antioxidant activity based on chitosan with the incorporation of sacha inchi leaves extract for food packaging applications as well as determined the DPPH antioxidant, total phenolic content (TPC) and total flavonoid content. The in vitro antioxidant activity was evaluated by 2,2-dipheny1-1-picrylhydrazyl (DPPH) radical scavenging activity, folin ciocalteu for total flavonoid content and aluminium chloride for total flavonoid content. The ICso value was presented where the value is decrease if the concentration of sacha inchi where added. Hence, 1.5% sacha inchi extract have the lowest of ICso value. Total phenolic content (TPC) test is also showed great value since p<0.05 and the highest concentration on gallic acid standard curve is 1.5% sacha inchi Extract (155.789 ± 4.218 GAE/mg). Other than that, total flavonoids content (TFC) also showed great concentration value since their value are also p<0.05 and the highest concentration on gallic acid standard curve is 1.5% sacha Inchi Extract (60.238 ± 1.5099 QE/mg). These findings offered a suggestion that the prepared chitosan films incorporated with sacha inchi extract can be applied as novel film in food packaging industry.