Optimization of ultrasonic - Assisted Enzymatic Extraction of Tannic Acid from Chromolaena Odorota sp.

A study was conducted by applying ultrasound-assisted enzymatic extraction (UAEE) to Chromolaena Odorota sp. to extract tannic acid as one of the phenolic compounds in the plant, undergo extraction with parameters which includes enzyme concentration, sonication time and duty cycle at constant temperature of 50 °C, solid to liquid ratio of 1:10 and sonication power at 60% amplitude. The optimum extraction process were found at cellulase enzyme concentration of 4%, sonication time of 60 minutes and duty cycle of 50% with the obtained concentration of tannic acid at 1.6152 mg/mL. The effectiveness of the extraction was further being proved by characterization of the extract which includes phytochemical screening, FTIR and SEM analysis. The blue-black coloration further prove that, there is presence of tannin in the sample. As for the FTIR, the functional groups found are correlated with the identified functional groups in tannic acid. The effect of cavitation and effect of enzyme-compound complex can be proven by presence of pore and pore size shown in SEM analysis. The study shows that the UAEE could be employed to enhance yield of tannic acid, reduce the extraction time and ensuring green extraction method were applied in the study.
Keywords: Tannic acid, Ultrasound-Assisted Enzymatic Extraction (UAEE), sonication time, duty cycle, enzyme concentration