Protein hydrolysis of black soldier fly larvae (BSFL) By using enzyme and fruit waste treatment

Black soldier fly larvae (BSFL), Hermetia Illucens L., thrive on organic waste, reducing environmental pollution, and converting waste material into insect-rich protein and fat. Thus, poultry feed formulations may help increase this insect species and make it one of the most capable for commercial production of BSFL's protein content. Furthermore, amino acid profiles are like various high-protein feedstuffs, such as fish meals and soybean meals. Nevertheless, only half of the protein content is being absorbed by the chicken gut. Thus, protein hydrolysis might be performed enzymatically to enhance broiler chicken digestibility and growth performance. In this work, multiple kinds of enzymes were utilized to hydrolyze protein in BSFL, including commercial protease and three types of fruit waste isolated from pineapple peel, bromelain, and date seed. The BSFL meal was treated with enzymes before the percentage of protein hydrolysis was determined. Three temperatures (30°C, 45°C, and 60°C) and four incubation times (0 hour, 1 hour, 3 hours, and 5 hours) were used to evaluate enzyme extracted from commercial protease, pineapple peel, bromelain, and date seed. The result reveals that pineapple peel (34%) has the highest percentage of protein reduction, followed by date seed (31%), bromelain (30%), and commercial protease (27%). The selected enzyme-treated BSFL was analysed using SDS-PAGE to prove this hydrolysis. This research demonstrates the ability of certain fruit waste to hydrolyze BSFL protein into smaller molecules. This discovery provides an opportunity for treated BSFL to become a low-cost and high-digestibility protein source for animal feed in the future.