Antibacterial activity determination of extract from leaf of Terminalia Catappa

Since the beginning of antibiotic era in 1928, numerous antibiotics have been found or synthesized from natural resources. Nowadays, many studies have been conducted intensively To tackle the antibiotic resistance dilemma that becoming increasing lately. Therefore, the purposes of this research were to study the antibacterial potential of five different extracts (hexane, chloroform, acetone, ethyl acetate, and methanol) of T. catappa leaves on selected Gram-positive and Gram-negative bacteria using disc diffusion assay and to preliminary screen the group of compound present in leaves extract of T. catappa. Result of the antibacterial activity showed that all of the extracts were able to inhibit Gram-positive and Gram-negative bacteria with zone inhibition ranging from 5.33 until 24.00 mm. However, acetone extracts of T. catappa showed better zone of inhibition, 24.00 mm against Bacillus cereus and Staphylococcus aureus compared to other extracts. Furthermore, chloroform, acetone, ethyl acetate, and methanol extracts of T. catappa revealed that the presence of secondary metabolites such as flavonoids, alkaloids, phenols, lactones, and anthraquinone through chemical profiling of antibacterial compound using TLC plates. In conclusion, acetone, ethyl acetate, and methanol extracts of T. catappa have the potential as a new source of antibacterial agent and can become a valuable source of novel medications due to their promising potential in antibacterial testing.