This study aims to identify the effects of fish meal substitution with Black Soldier Larvae (BSFL) and earthworm on growth and physiological properties of experimental diets in betta fish (Betta Splendens) production. The feed cost can rise by more than 70% of production because the soybean and fish meal are costly. The replacement of Black Soldier Fly Larvae (BSFL) and earthworm in feed ingredient can cut off the feed cost. Both BSFL and earthworm contain the nutrient composition that meets the requirement of fish feed. The excellent quality of feeds will determine the performance and survival rate of the fish. However, many factors can affect the development of the Betta Splendens, such as the substrate of nutrition, water quality, pathogen and stress. (R, N, & W., 2016). Two types of the feeding trial, BSFL and earthworm was used to determine the better survival rate and organoleptic of the water of Betta Fish (Betta Splendens).