Cellulose enzyme activity from Trichoderma Reesel extract using Hexane solvent

Trichoderma is a genus of a soil-borne fungus with a long history of being economically important in agriculture. This filamentous genus species are mainly used as bio fungicides and plant growth modifiers. Therefore, this study was conducted to look at the enzymatic activity of cellulose from T.reesei species. The focuses on enzymatic activity of cellulose because of the problem for cellulose enzyme industry such as synthetic enzyme preparation cost. The investigation started with sample collection, followed by Potato Dextrose Agar (PDA) and Potato Dextrose Broth (PDB) preparation, T.reesei isolation, actual culture, and T.reesei morphological observation. Then, the extraction of secondary metabolites from Trichoderma Reesei. The isolation process is performed using a separator funnel. Next, a rotary evaporator machine was used to extract the solution with a boiling point of 69°C until it was dry. After that, the extract was stored under a fume hood for air drying for five days. To determine the cellulolytic activity Jensen Media (JM) was used. Sterile filter paper discs (15.7 mm) were immersed in a control solution at different concentrations of 5 mg, 10 mg, 15 mg, and 20 mg of extractor solution. It was found that there was a significant difference in the diameter of the halo zone of each treatment. T.reesei produced the minor diameter of halo zone is Treatment 2 (10 mg/5m1) is 20 mm. For the Treatment 3 (15 mg/5m1) and Treatment 4 (20 mg/5 ml) were found forming larger clearing halo zones from 50 mm to 60 mm after straining with Congo red. Trichoderma species must be accurately determined because these strains can be employed as a biocontrol agent to prevent disease and boost yield in agriculture crops. Keywords: Trichoderma reesei, enzymatic activity of cellulose, secondary metabolites, Jensen Media (JM), diameter of halo zone.