Antibiogram of Edwardsiella Tarda isolated from diseased Anglefish (Pterophyllum Scalare) of aquarium shop

The purpose of the present study was isolate and identified Edwardsiella tarda from diseased Pterophyllum scalare of aquarium shop. E.tarda is a gram-negative bacterium with facultative properties and will lead to several diseases in fish, human, and other animal. E.tarda can be found in fish and ornamental fish by isolate from freshwater or brackish water environments. It has also been isolated from the human stool after consuming freshwater food source such as catfish, eel and including reptiles. Therefore, this is crucial ornamental fish are rare in intensive aquaculture activity. Despite all the previous research, the study of E.tarda already has antibiotic yet it still not be commercialize and still under developing. In this research, diseased Pterophyllum scalare, anglefish were collected from aquarium shop in Jeli, Kelantan and lesion were swab on the external and internal of the ornamental fish at the abdomen, skin, gills, eyes, and kidney. The method of isolation E.tarda was used Xylose-Lysine-Deoxycholate (XLD) agar follow with identification by using BBL Crystal commercial kit. The selected bacteria were subjected to diffusion method. This 30 of isolates E. tarda was then continued into Antibiogram disk that were used in the present study. At the mean time the sensitivity of E.tarda was determined the characterize antibiogram with 16 types of antimicrobial susceptibility test. The result was analyzed convert as Sensitive (S), Intermediate Sensitive (IS) or Resistance (R) by using Antibiotic resistance index and MAR calculation. The antibiogram of isolates was found to be highly sensitive to flumequine and resistance to amoxycillin antibiotic. This finding is indicate that E.tarda presence of combination of the antibiotic resistance in angelfish isolates and has the possibility found in others ornamental fish.