Enzymatic degradation of lignin by Trichoderma Reesei extract using ethyl acetate solvent

Lignocellulosic biomass (LCB) is indeed a renewable asset made up mostly of cellulose and hemicelluloses (polysaccharides) and an aromatic polymer derived from plants (lignin). Considering lignocellulosic biomass is the most abundant residual from agricultural production, it has been given major attention as a source of biomass for biofuel production. Converting lignocellulosic biomass to biofuels for transportation fuels is a viable strategy for boosting energy security while reducing the impact on the environment such as air pollutants and emissions of greenhouse gas. Since the demand and consumption of fuel is rising every year, fast action must be taken to increase the efficiency of biofuel production from the lignocellulosic residual. Besides, the creation of second-generation biofuel production from lignocellulosic waste has numerous benefits from both environmental and energy aspects. However, the expense of cellulase enzymes is a significant component to the operating costs of a biorefinery that produces biofuel from lignocellulosic feedstock. Trichoderma species is known as lignocellulolytic fungi and has high potential in degrading plant biomass through cellulolytic activity. This fungi species also has the ability to manufacture various enzyme that can be used to break down cellulose, starch and lipid. Trichoderma reesei is among Trichoderma species that became main producer of lignocellulolytic enzyme and T reesei have a developed cellulase enzyme system that is persistent, extracellular, and productive. T.reesei owing to its effectiveness in the synthesis of cellulose- and starch-degrading enzymes in much research that has been conducted. The experiment in this research is conducted to see the degradation activity of lignin by Trichoderma reesei extract with different ration using ethyl acetate solvent. The concentration of T.reesei used is 5 mg, 10 mg, 15 mg and 20 mg. The enzymatic degradation of lignin can be seen on 10 mg, 15 mg and 20 mg concentration of T.reesei extract which the diameter of dark brown halo zone Increase while the concentration is higher.