Introduction of new weed management in crops production are required since current weed control strategy is less effective and highly herbicide dependent, thus leading to development of herbicide resistance weeds as well as environmental and human health concerns. This study aimed to evaluate phytotoxic effects ofN fertilizer (urea) treated with natural plant oil herbicide (lemon grass) for barnyard grass (Echinochloa crus-galli) control at the vegetative stage. The bioassay weed species was treated with a series application rate of urea (80, 160, and 240 kg had) and natural plant oil herbicide (0.01, 0.02, and 0.04 g/L). The results in current study have shown that natural plant oil herbicide at 0.04 g/L in combination with urea at rate of 80 kg ha (T7) strongly inhibited E. truss galb emergence by 90 %. Meanwhile, starting from 0.02 to 0.04 g/L natural plant oil herbicide with 80 to 240 kg ha-I urea (T4-T9), the shoot fresh weight of E. crus-galli was significantly reduced by 67-94% inhibition. At these similar application rates, the root length of E. crus-galli was very susceptible to natural plant oil herbicide and urea fertilizer where the treated bioassay species shows 54-78% reduction in root length. The results of this study suggest that regardless of any application rate, the integration of natural plant oil herbicide provide effective weed control by increase the phytotoxicity of urea for weed inhibition.