An abstract of the research paper presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement on the course DVT 55204 – Research Project. Oscillatoria spp. is a cyanophyceae commonly found in both fresh and marine waters of Malaysia. It contains bioactive compounds such as flavonoids, phenolics, and terpenoids that are said to have antimicrobial properties. In this study, Oscillatoria spp. collected from Gunung Stong, Jeli, Kelantan, was cultured using four different water mediums to observe its growth. Extraction using ethanol and dimethyl sulfoxide (DMSO) was done to perform an antimicrobial assay against Aeromonas hydrophila and Streptococcus agalactiae. Oscillatoria spp. was present in all 15 samples collected from the river. Also, it was observed that the algae was able to grow better in fish tank water and soil water as compared to tap water and distilled water. Next, the antimicrobial assay of the DMSO and ethanolic extract of Oscillatoria spp. against S. agalactiae and A. hydrophila showed bacterial resistance. Multiple factors of the unsuccessful antimicrobial assay includes the usage of raw extract of Oscillatoria spp., agar well diffusion error and the unsuitability of procedure respective to its extracts.
Keywords: Oscillatoria spp., Aeromonas hydrophila, Streptococcus agalactiae, Antimicrobial assay.