The effect of ethanolic plant extract, centella asiatica against salmonella typhi and streptococcus agalactiae

Low-growing pan-tropical perennial Centella asiatica. It spreads and forms a dense ground cover, which is advantageous in some situations. Salmonella is one of the world's deadliest food-borne infections. Streptococcus agalactiae causes chronic, infectious cow mastitis, as well as invasive sickness in camels, dogs, cats, fish, and hamsters. This study examined the effect of ethanolic plant extract on Salmonella typhi and Streptococcus agalactiae. Centella asiatica was purchased from a wet market and stored in zip-lock bags. Allow plant samples to air dry at room temperature for three days, then grind them into a fine powder. Then, it will be soaked in ethanol with 1:100 ratio and left to rest at room temperature before filtering the extracts and concentrating the filtrate under decreasing pressure for 15 minutes at 40˚ C. Salmonella typhi and Streptococcus agalactiae have been cultivated on selective media: nutrient agar for S. agalactiae and Xylose Lysine Deoxycholate agar for S. typhi. For the antimicrobial disc diffusion test for plant extracts, the material was diluted with DMSO. The disc was placed on MHA agar already streaked with bacteria and incubated for 24 hours. The plant extract shows no inhibitory zone on bacteria. At 62.5 mg/ml, 125 mg/ml, 250 mg/ml, 500 mg/ml, and 1000 mg/ml, there is no inhibitory zone on the bacteria S. typhi and S. agalactiae. No inhibition zone may be related to the morphological nature of bacteria, whose cell walls have numerous layers that can limit the extract's antibacterial activity and cause resistance to plant extracts. Antimicrobial activity was dose-dependent, and extraction solvent affected antibacterial metabolites. Antibacterial tests of C. asiatica showed that ethanol extracts were ineffective against