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In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6.


Zeti Ermiena Surya Mat Hussin (2016) In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6. Masters thesis, Universiti Malaysia Kelantan.


As an important fiber crop, many potential applications of kenaf are being identified and developed in 21 century, especially in developed countries such as America, Japan, and France and Malaysia as well. The present study report a protocol for the efficient in vitro propagation of kenaf (H.cannabinus L) to initiate multiple shoot from mother plant part (shoot, petiole, node and leaf) through direct and indirect organogenesis using MS medium + BAP + IAA for direct organogenesis and MS medium + KN+2,4-D for indirect organogenesis to get callus and after 8 weeks, the calli were put in the MS medium + BAP +IAA for shoot induction. The highest number of shoots produced from node explants part via direct organogenesis (16.33/explants) in MS medium + 0.5 mg/l BAP +0.05 mg/l IAA. The highest percentage explants forming callus and callus generate into shoot also from node explants part which was induced 75% callus from explants and 73.33% of callus turn into shoot in MS medium + 0.1 mg/l BAP. Several subcultures were drived in order to enhance the multiplication rate. The treatments have their significant different with others. The shoots then were transferred to the root induction medium. Shoots showed the vigorous roots in the MS basal medium. The in vitro rooted plantlets were acclimatized in sand+ coco pit + vermiculite with ratio 3:2:2 and were covered with hole container for 0-15 days to test the effect of humidity to plantlets. The 9th-15th days explants covered with container showed 100% of survive. Survive well rooted plantlets were transferred to the field. Plants grew well into maturity without any remarkable morphological variations within the treatments.

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Additional Metadata

Item Type: UMK Etheses
Collection Type: Thesis
Date: 2016
Number of Pages: 110
Supervisor: Dr. Dwi Susanto
Programme: Master of Science
Institution: Universiti Malaysia Kelantan
Subjects: Q Science > QK Botany
Faculty/Centre/Office: Faculty of Agro - Based Industry
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